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Purification of Ubiquitinated p53 Proteins from Mammalian Cells

2.0K Views

10:55 min

March 21st, 2022

DOI :

10.3791/63602-v

March 21st, 2022


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Ubiquitinated P53 Proteins

Chapters in this video

0:05

Introduction

0:43

Plasmid Transfection

2:16

Cell Collection

2:52

Purification of Ubiquitinated Proteins under Nondenaturing Conditions

5:01

Purification of Ubiquitinated Proteins under Denaturing Conditions

7:49

Detection of Purified Ubiquitinated Proteins by Western Blotting

8:29

Results: Ubiquitinated p53 Proteins Purified under Nondenaturing and Denaturing Conditions

10:22

Conclusion

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