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Amsterdam Cardiovascular Sciences

3 ARTICLES PUBLISHED IN JoVE

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JoVE Core

Simultaneous Electrical and Mechanical Stimulation to Enhance Cells' Cardiomyogenic Potential
Aida Llucià-Valldeperas 1,2, Ramon Bragós 3, Antoni Bayés-Genís 1,4,5,6
1Insuficiencia Cardiaca y Regeneración Cardiaca (ICREC) Research Program, Health Science Research Institute Germans Trias i Pujol, 2Amsterdam Universitair Medisch Centrum (UMC), Vrije Universiteit Amsterdam, Pulmonology and Physiology, Amsterdam Cardiovascular Sciences, 3Electronic and Biomedical Instrumentation Group, Departament d'Enginyeria Electrònica, Universitat Politècnica de Catalunya, 4Cardiology Service, Germans Trias i Pujol University Hospital, 5Department of Medicine, Universitat Autònoma de Barcelona, 6Centro de Investigación Biomédica en Red (CIBER) Cardiovascular, Instituto de Salud Carlos III

Here we present a protocol for training a cell population using electrical and mechanical stimuli emulating cardiac physiology. This electromechanical stimulation enhances the cardiomyogenic potential of the treated cells and is a promising strategy for further cell therapy, disease modeling, and drug screening.

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Biology

Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Rafeeh Dinani 1,2, Emmy Manders 1,2,3, Michiel Helmes 1,2,3, Lili Wang 4, Bjorn C. Knollmann 4, Diederik W. D. Kuster 1,2, Jolanda van der Velden 1,2
1Division of Physiology, Amsterdam UMC, Vrije University, 2Heart Failure & Arrhythmias, Amsterdam Cardiovascular Sciences, 3CytoCypher BV, 4Division of Clinical Pharmacology, Vanderbilt School of Medicine

Here, an established method is described to perform contractility and calcium measurements in human induced pluripotent stem cell-derived cardiomyocytes using an optics-based platform. This platform enables researchers to study the effect of mutations and the response to various stimuli in a rapid and reproducible manner.

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Medicine

Induction of Cerebral Arterial Gas Embolism in Rat
Raoul A. Fakkert 1,2,3, Nina C. Weber 3, Benedikt Preckel 1,3, Robert A. van Hulst 1,2, Inge A. Mulder *4,5,6, Robert P. Weenink *1,2
1Department of Anesthesiology, Amsterdam UMC, 2Department of Hyperbaric Medicine, Amsterdam UMC, 3Laboratory of Experimental Intensive Care and Anesthesiology, Amsterdam UMC, 4Biomedical Engineering and Physics, Amsterdam UMC, 5Microcirculation, Amsterdam Cardiovascular Sciences, 6Neurovascular Disorders, Amsterdam Neurosciences

This protocol provides a detailed description of inducing cerebral air emboli in rats. It compares direct injection into the common carotid artery and introduction through the external carotid artery. It provides a technical description of the air bubble generator, the effect of different air volumes, and procedural challenges.

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