Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

2.7K Views

•

06:42 min

•

May 26th, 2023

DOI :

10.3791/65326-v

May 26th, 2023

•

Rafeeh Dinani¹^,², Emmy Manders¹^,²^,³, Michiel Helmes¹^,²^,³, Lili Wang⁴, Bjorn C. Knollmann⁴, Diederik W. D. Kuster¹^,², Jolanda van der Velden¹^,²

¹Division of Physiology, Amsterdam UMC, Vrije University, ²Heart Failure & Arrhythmias, Amsterdam Cardiovascular Sciences, ³CytoCypher BV, ⁴Division of Clinical Pharmacology, Vanderbilt School of Medicine

Summary

Explore More Videos

Real time Measurements

Calcium Handling

Contractility Parameters

Human Induced Pluripotent Stem Cells

Cardiomyocytes

Calcium sensitive Fluorophore

Pixel Correlation

Intracellular Calcium Transients

Drug Screening

Functional Parameters

Contraction Kinetics

Preclinical Research

Hyperswitch

Ratiometric Measurements

2D Assessment

Chapters in this video

0:00

Introduction

1:13

Contractility Measurements in hiPSC‐CMs

3:39

Calcium Transient Measurements in hiPSC‐CMs

5:04

Contractility and Calcium Transient Measurements Data Analysis

Related Videos

12:10

Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set

29.8K Views

10:24

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells

21.4K Views

10:46

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

19.4K Views

13:23

Selecting and Isolating Colonies of Human Induced Pluripotent Stem Cells Reprogrammed from Adult Fibroblasts

19.8K Views

12:10

Measuring Fast Calcium Fluxes in Cardiomyocytes

15.1K Views

08:25

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP

20.4K Views

13:18

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells

21.2K Views

09:43

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

22.3K Views

09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

22.6K Views

13:13

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry

30.1K Views