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Texas A&M University (TAMU)

10 ARTICLES PUBLISHED IN JoVE

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Biology

Method for Culture of Early Chick Embryos ex vivo (New Culture)
Delphine Psychoyos 1, Richard Finnell 2
1Center for Environmental and Genetic Medicine, Institute of Biosciences and Technology - Texas A&M Health Science Center , 2Center for Environmental and Genetic Medicine, Texas A&M University (TAMU)

This video demonstrates New culture, a method by which chick embryos are cultured outside the egg for up to 24 hr. This method enables one to study early development (primitive streak to 14 som.), a period corresponding to E7-9 in mouse. Applications of this technique include electroporation, in situ hybridization and immunohistochemistry.

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Biology

Double Whole Mount in situ Hybridization of Early Chick Embryos
Delphine Psychoyos 1, Richard Finnell 2
1Center for Environmental and Genetic Medicine, Institute of Biosciences and Technology - Texas A&M Health Science Center , 2Center for Environmental and Genetic Medicine, Texas A&M University (TAMU)

This video demonstrates 2-color whole mount in situ hybridization, a method by which the spatial and temporal expression pattern of 2 different genes can be visualized in young chick embryos. This method was originally introduced by David Wilkinson, Domingos Henrique, Phil Ingham and David Ish -Horowicz.

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Biology

Method for Whole Mount Antibody Staining in Chick
Delphine Psychoyos 1, Richard Finnell 1
1Institute of Biosciences and Technology, Center for Environmental and Genetic Medicine, Texas A&M University (TAMU)

This video demonstrates whole mount immunohistochemistry, a method by which the spatial and temporal expression pattern of an antigen can be visualized in young chick embryos. This method was originally introduced by Jane Dodd and Tom Jessell.

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Biology

Assay for Neural Induction in the Chick Embryo
Delphine Psychoyos 1, Richard Finnell 1
1Institute of Biosciences and Technology, Center for Environmental and Genetic Medicine, Texas A&M University (TAMU)

Neural induction is the first step in the formation of the brain. It is a mechanism by which Hensen's node (organizer), instructs adjacent tissue to adopt a neural fate, i.e. to give rise to the nervous system. This video demonstrates an assay for neural induction in chick embryo.

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Biology

Preparation of Rat Brain Aggregate Cultures for Neuron and Glia Development Studies
Hisami Koito 1, Jianrong Li 1
1Department of Veterinary Integrative Biosciences, Texas A&M University (TAMU)

A protocols for an embryonic rat brain aggregate culture system is described. Multipotent progenitors in the aggregates can develop and differentiate into neurons, astrocytes and oligodendrocytes.

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Biology

Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules
John Noel 1, Winfried Teizer 1, Wonmuk Hwang 2
1Department of Physics, Texas A&M University (TAMU), 2Department of Biomedical Engineering, Texas A&M University (TAMU)

We present a procedure for forming a poly(ethylene glycol) self-assembled monolayer (PEG-SAM) on a silicon substrate with gold microelectrodes. The PEG-SAM is formed in a single step and prevents biofouling on silicon and gold surfaces. Electrophoresis is then used for patterning biomolecules down to the nanoscale.

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Biology

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform
Jaewon Park 1, Hisami Koito 2, Jianrong Li 2, Arum Han 1
1Department of Electrical and Computer Engineering, Texas A&M University (TAMU), 2Department of Veterinary Integrative Biosciences, Texas A&M University (TAMU)

We developed a novel multi-compartment neuron co-culture microsystem platform for in vitro CNS axon-glia interaction research. The platform is capable of conducting up to six independent experiments in parallel and was fabricated using a newly developed macro/micro hybrid fabrication method.

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Neuroscience

Automated Interactive Video Playback for Studies of Animal Communication
Trisha Butkowski 1, Wei Yan 1, Aaron M. Gray 2, Rongfeng Cui 2, Machteld N. Verzijden 2, Gil G. Rosenthal 2
1Department of Visualization, Texas A&M University (TAMU), 2Department of Biology, Texas A&M University (TAMU)

Video playback is a widely used technique in animal behavior. We created and evaluated a program that applies rules-based, interactive playback of 3-D computer animations in response to real-time, automated data on subject behavior.

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Immunology and Infection

A Calcium Bioluminescence Assay for Functional Analysis of Mosquito (Aedes aegypti) and Tick (Rhipicephalus microplus) G Protein-coupled Receptors
Hsiao-Ling Lu 1, Cymon N. Kersch 1, Suparna Taneja-Bageshwar 1,2, Patricia V. Pietrantonio 1
1Department of Entomology, Texas A&M University (TAMU), 2Department of Molecular and Cellular Medicine, Texas A&M University (TAMU)

This protocol provides instructions for clonal-cell line selection and a calcium bioluminescence assay to analyze the structure-activity relationships of synthesized arthropod neuropeptides on their cognate GPCRs. This assay can be used for receptor deorphanization and structure-activity relationship studies for synthetic analog design and peptide/drug-lead discovery.

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Biochemistry

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors
Lin Cheng *1, Lantian Su *1, Xiaowen Tian *1, Fan Xia 2, Chang Zhao 1, Wei Yan 1, Zhenhua Shao 1
1Division of Nephrology and Kidney Research Institute, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, 2Department of Neurosurgery, West China Hospital, Sichuan University

S1P exerts its diverse physiological effects through the S1P receptors (S1PRs) subfamily. Here, a pipeline is described to expound on the structures and function of S1PRs.

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