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2 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry
Christina Farr 1, Stuart Berger 1,2
1Immunology, University of Toronto, 2Arthritis and Immune Disorder Research Centre, University Health Network (UHN)

This article will detail the protocol for measuring calpain activity in fixed and living cells using flow cytometry.

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Immunology and Infection

Isolation and Identification of Extravascular Immune Cells of the Heart
Laura Aronoff 1,2, Slava Epelman 1,2,3,4,5, Xavier Clemente-Casares 1,2
1Toronto General Hospital Research Institute, University Health Network (UHN), 2Dept of Laboratory Medicine and Pathobiology, University of Toronto, 3Dept of Immunology, University of Toronto, 4Peter Munk Cardiac Centre, 5Ted Rogers Centre for Heart Research

This protocol presents a simple and efficient method to isolate, identify and quantify immune cells residing in the myocardium of mice during steady state or inflammation. The protocol combines enzymatic and mechanical digestion for the generation of a single cell suspension that can be further analyzed by flow cytometry.

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