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Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry

11.8K Views

11:37 min

July 8th, 2010

DOI :

10.3791/2050-v

July 8th, 2010


Transcript

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Calpain Activity

Chapters in this video

0:06

Title

0:48

Introduction

1:15

Reagent Preparation

1:56

Cell Preparation

3:11

Calpain Assay in Fixed Cells

4:48

Data Acquisition (Fixed Cells)

6:55

Calpain Assay in Live Cells

8:22

Calpain Assay in Mixed Cells

10:05

Analysis and Representative Results

11:09

Conclusion

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