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Institució Catalana de Recerca i Estudis Avançats (ICREA)

4 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Intravital Microscopy of the Spleen: Quantitative Analysis of Parasite Mobility and Blood Flow
Mireia Ferrer *1, Lorena Martin-Jaular *1, Maria Calvo 2, Hernando A. del Portillo 1,3
1Department of poverty related diseases, Barcelona Centre for International Health Research, 2Confocal Microscopy Unit, University of Barcelona- Scientific and Technological Centers, 3Institució Catalana de Recerca i Estudis Avançats (ICREA)

We show the method for performing intravital microscopy of the spleen using GFP transgenic malaria parasites and the quantification of parasite mobility and blood flow within this organ.

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Bioengineering

Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation
Ignasi Casanellas 1,2, Anna Lagunas 3,1, Iro Tsintzou 1, Yolanda Vida 4,5, Daniel Collado 4,5, Ezequiel Pérez-Inestrosa 4,5, Cristina Rodríguez-Pereira 6, Joana Magalhaes 3,6, Pau Gorostiza 1,3,7, José A. Andrades 8,3, José Becerra 8,3,5, Josep Samitier 1,3,2
1Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), 2Department of Engineering Electronics, University of Barcelona (UB), 3Networking Biomedical Research Center (CIBER), 4Instituto de Investigacin Biomédica de Málaga (IBIMA), Department of Organic Chemistry, Universidad de Málaga (UMA), 5Andalusian Centre for Nanomedicine and Biotechnology-BIONAND, 6Unidad de Bioingeniería Tisular y Terapia Celular (GBTTC-CHUAC), Grupo de Reumatolog ía, Instituto de Investigación Biomèdica de A Coruña (INIBIC), Complexo Hospitalario Universitario de A Coruña (CHUAC), Sergas, Universidade da Coruña (UDC), 7Institució Catalana de Recerca i Estudis Avançats (ICREA), 8Instituto de Investigación Biomédica de Málaga (IBIMA), Department of Cell Biology, Genetics and Physiology, Universidad de Málaga (UMA)

A method to obtain dendrimer-based uneven nanopatterns that permit the nanoscale control of local arginine-glycine-aspartic acid (RGD) surface density is described and applied for the study of cell adhesion and chondrogenic differentiation.

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Neuroscience

Calcium Imaging In Electrically Stimulated Flat-Mounted Retinas
Marina Cunquero 1, Maria Marsal 1, Gustavo Castro-Olvera 1, Steven T. Walston 2, F. Taygun Duvan 2, José Gabriel Macias-Montero 3, Carles Puigdengoles 3, Mokhtar Chmeissani 3, Jose A. Garrido 2,4, Pablo Loza-Alvarez 1
1Institut de Ciències Fotòniques (ICFO), The Barcelona Institute of Science and Technology, 2Catalan Institute of Nanoscience and Nanotechnology (ICN2), CSIC and BIST, Campus UAB, 3Institut de Física d'Altes Energies (IFAE), The Barcelona Institute of Science and Technology, Campus UAB, 4Institució Catalana de Recerca i Estudis Avançats (ICREA)

Retinal prostheses have the ability to generate visual perceptions. To advance the development of new prostheses, ex vivo methods are needed to test devices before implantation. This article provides a comprehensive protocol for studying calcium activity in the retinal ganglion cell layer when subjected to electrical stimulation.

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Medicine

Non-Invasive Monitoring of Microvascular Oxygenation and Reactive Hyperemia using Hybrid, Near-Infrared Diffuse Optical Spectroscopy for Critical Care
M. Atif Yaqub *1, Marta Zanoletti *1, Lorenzo Cortese 1, Daniel Senciales Sánchez 1, Caterina Amendola 2, Lorenzo Frabasile 2, Umut Karadeniz 1, Jacqueline Martinez Garcia 1, Marta Martin 1, Jordi Cortes-Picas 1, Alba Caballer 3, Edgar Cortes 3, Sara Nogales 3, Alberto Tosi 4, Talyta Carteano 5, Diego Sanoja Garcia 5, Jakub Tomanik 6, Tessa Wagenaar 6, Hsiao Mui 6, Claudia Nunzia Guadagno 7, Shahrzad Parsa 8, Sanathana Konugolu Venkata Sekar 7, Luc Demarteau 6, Tijl Houtbeckers 6, Udo M. Weigel 8, Michele Lacerenza 9, Mauro Buttafava 9, Alessandro Torricelli 2,10, Davide Contini 2, Jaume Mesquida 3, Turgut Durduran 1,11
1ICFO - Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology, 2Dipartimento di Fisica, Politecnico di Milano, 3Critical Care Department, Parc Taulí Hospital Universitari, 4Dipartimento di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, 5ASPHALION s.l., 6Splendo, 7BioPixS-Biophotonics Standards, IPIC, Tyndall National Institute, 8Hemophotonics s.l., 9PIONIRS s.r.l., 10Consiglio Nazionale delle Ricerche, Istituto di Fotonica e Nanotecnologie, 11Institució Catalana de Recerca i Estudis Avançats (ICREA)

We describe a protocol to non-invasively and continuously measure absolute microvascular blood flow index and blood oxygen saturation using a multi-modal device based on near-infrared diffuse optics. We then evaluate the metabolic rate of oxygen consumption and reactive hyperemia utilizing a vascular occlusion test.

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