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Ecole Polytechnique Fédérale de Lausanne (EPFL)

5 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
Melanie Blokesch 1
1Global Health Institute, School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL)

A quick method to modify the genome of V. cholerae is described. These modifications include the deletion of single genes, gene clusters and genomic islands as well as the integration of short sequences (e.g. promoter elements or affinity-tag sequences). The method is based on the natural transformation and FLP-recombination.

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Engineering

Nanomoulding of Functional Materials, a Versatile Complementary Pattern Replication Method to Nanoimprinting
Corsin Battaglia 1,2, Karin Söderström 1, Jordi Escarré 1, Franz-Josef Haug 1, Matthieu Despeisse 1, Christophe Ballif 1
1Institute of Microengineering (IMT), Photovoltaics and Thin Film Electronics Laboratory, Ecole Polytechnique Fédérale de Lausanne (EPFL), 2Department of Electrical Engineering and Computer Sciences, University of California, Berkeley

We describe a nanomoulding technique which allows low-cost nanoscale patterning of functional materials, materials stacks and full devices. Nanomoulding can be performed on any nanoimprinting setup and can be applied to a wide range of materials and deposition processes.

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JoVE Journal

Preparation of Carbon Nanosheets at Room Temperature
Stephen Schrettl 1, Bjoern Schulte 1, Cristina Stefaniu 2, Joana Oliveira 2, Gerald Brezesinski 2, Holger Frauenrath 1
1Institute of Materials, Ecole Polytechnique Fédérale de Lausanne (EPFL), 2Colloid Chemistry Department, Max Planck Institute of Colloids and Interfaces

We present the synthesis of an amphiphilic hexayne and its use in the preparation of carbon nanosheets at the air-water interface from a self-assembled monolayer of these reactive, carbon-rich molecular precursors.

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Medicine

Improvement of a Closed Chest Porcine Myocardial Infarction Model by Standardization of Tissue and Blood Sampling Procedures
Mai M. Abdelhafez 1,2, Jane Shaw 1, Jonas Wilbs 3, Alain Despont 1, Robert Rieben 1
1Department for Biomedical Research, University of Bern, 2Graduate School for Cellular and Biomedical Sciences, University of Bern, 3Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne (EPFL)

Here we demonstrate a protocol to standardize sampling procedures of an established porcine model of acute myocardial infarction in order to increase its translational value in the understanding of the pathophysiology of myocardial ischemia/reperfusion injury and to test novel drug candidates.

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Biochemistry

Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy
Andreas Reif 1, Anass Chiki 1, Jonathan Ricci 1, Hilal A. Lashuel 1
1Laboratory of Molecular and Chemical Biology of Neurodegeneration, Ecole Polytechnique Fédérale de Lausanne (EPFL)

Here, we present a robust and optimized protocol for the production of milligram quantities of native, tag-free monomers and fibrils of the exon1 of the Huntingtin protein (Httex1) based on the transient fusion of small ubiquitin related modifier (SUMO).

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