Centre de Recherche en Cancérologie de Lyon

3 ARTICLES PUBLISHED IN JoVE

Biology

Isolation and Culture of Mouse Primary Pancreatic Acinar Cells

Johann Gout^1,2,3,4,5, Roxane M. Pommier^1,2,3,4,5, David F. Vincent^1,2,3,4,5, Bastien Kaniewski^1,2,3,4,5, Sylvie Martel^1,2,3,4,5, Ulrich Valcourt^*1,2,3,4,5, Laurent Bartholin^*1,2,3,4,5

¹INSERM U1052, Centre de Recherche en Cancérologie de Lyon, ²CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, ³Université de Lyon, ⁴Université Lyon 1, ⁵Centre Léon Bérard

In this publication, we describe a rapid and convenient procedure for isolating and culturing primary pancreatic acinar cells from the murine pancreas. This method constitutes a valuable approach to study the physiology of fresh primary normal/untransformed exocrine pancreatic cells.

Cancer Research

Proximity Ligation Assay Allows the Detection, Localization, and Quantification of Protein Arginine Methylation in Fixed Tissue

Coralie Poulard^*1,2,3, Julien Jacquemetton^*1,2,3, Julie Valantin^1,2,3,4,5, Isabelle Treilleux^1,2,3,4, Muriel Le Romancer^1,2,3

¹Université de Lyon, ²Inserm U1052, Centre de Recherche en Cancérologie de Lyon, ³CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, ⁴Pathology Department, Centre Leon Bérard, ⁵Plateforme Anatomopathologie Recherche

Proximity ligation assay is a very useful technique to localize and quantify arginine methylation of a given protein when the modified arginine residue is unknown and/or if no specific antibody is available.

Biology

A Human Bone Marrow 3D Model to Investigate the Dynamics and Interactions Between Resident Cells in Physiological or Tumoral Contexts

Kawtar Arizkane^1,2,3, Kevin Geistlich^1,2,3,4, Laurine Moindrot^1,2,3, Emma Risson^1,2,3, Sandrine Jeanpierre^1,2,3,4, Léa Barral^1,2,3, Anaëlle Bobard^1,2,3, Giulio Menegazzi⁵, Thibault Voeltzel^1,2,3, Véronique Maguer-Satta^1,2,3, Sylvain Lefort^1,2,3

¹Centre de Recherche en Cancérologie de Lyon, ²Centre de Recherche en Cancérologie de Lyon, ³Université de Lyon, ⁴Centre Léon Bérard, ⁵Department of Experimental and Clinical Biomedical Sciences Mario Serio, Università degli Studi di Firenze

Here, we describe an easy-to-implement, standardized, microphysiological system that reflects the complexity of the human bone marrow's in vivo structure, providing a pertinent model to finely study a broad range of normal and pathological events.