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Isolation and Culture of Mouse Primary Pancreatic Acinar Cells

DOI :

10.3791/50514-v

August 13th, 2013

August 13th, 2013

34,622 Views

1INSERM U1052, Centre de Recherche en Cancérologie de Lyon, 2CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, 3Université de Lyon, 4Université Lyon 1, 5Centre Léon Bérard

In this publication, we describe a rapid and convenient procedure for isolating and culturing primary pancreatic acinar cells from the murine pancreas. This method constitutes a valuable approach to study the physiology of fresh primary normal/untransformed exocrine pancreatic cells.

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Keywords Mouse

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