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Centre Léon Bérard

3 ARTICLES PUBLISHED IN JoVE

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Biology

Isolation and Culture of Mouse Primary Pancreatic Acinar Cells
Johann Gout 1,2,3,4,5, Roxane M. Pommier 1,2,3,4,5, David F. Vincent 1,2,3,4,5, Bastien Kaniewski 1,2,3,4,5, Sylvie Martel 1,2,3,4,5, Ulrich Valcourt *1,2,3,4,5, Laurent Bartholin *1,2,3,4,5
1INSERM U1052, Centre de Recherche en Cancérologie de Lyon, 2CNRS UMR5286, Centre de Recherche en Cancérologie de Lyon, 3Université de Lyon, 4Université Lyon 1, 5Centre Léon Bérard

In this publication, we describe a rapid and convenient procedure for isolating and culturing primary pancreatic acinar cells from the murine pancreas. This method constitutes a valuable approach to study the physiology of fresh primary normal/untransformed exocrine pancreatic cells.

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Cancer Research

Evaluation of the Feasibility, Safety, and Accuracy of an Intraoperative High-intensity Focused Ultrasound Device for Treating Liver Metastases
Aurélien Dupre 1,2, David Melodelima 2, David Perol 3, Yao Chen 1, Jérémy Vincenot 2, Jean-Yves Chapelon 2, Michel Rivoire 1,2
1Department of Surgical Oncology, Centre Léon Bérard, 2Applications des ultrasons à la thérapie (LabTAU), Institut national de la santé et de la recherche médicale (INSERM), Centre Léon Bérard, Université Lyon 1, Université Lyon, 3Biostatistics and Treatment Evaluation Unit, Centre Léon Bérard

Here, we present an ablate-and-resect prospective study to evaluate the feasibility, safety, and accuracy of intraoperative high-intensity focused ultrasound ablation in patients undergoing hepatectomy for liver metastases.

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Biology

A Human Bone Marrow 3D Model to Investigate the Dynamics and Interactions Between Resident Cells in Physiological or Tumoral Contexts
Kawtar Arizkane 1,2,3, Kevin Geistlich 1,2,3,4, Laurine Moindrot 1,2,3, Emma Risson 1,2,3, Sandrine Jeanpierre 1,2,3,4, Léa Barral 1,2,3, Anaëlle Bobard 1,2,3, Giulio Menegazzi 5, Thibault Voeltzel 1,2,3, Véronique Maguer-Satta 1,2,3, Sylvain Lefort 1,2,3
1Centre de Recherche en Cancérologie de Lyon, 2Centre de Recherche en Cancérologie de Lyon, 3Université de Lyon, 4Centre Léon Bérard, 5Department of Experimental and Clinical Biomedical Sciences Mario Serio, Università degli Studi di Firenze

Here, we describe an easy-to-implement, standardized, microphysiological system that reflects the complexity of the human bone marrow's in vivo structure, providing a pertinent model to finely study a broad range of normal and pathological events.

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