Institut de Science et d'Ingénierie Supramoléculaires (ISIS), CNRS and Université de Strasbourg

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening

Viktoria Wollrab^1,2, David Caballero^1,2, Raghavan Thiagarajan^1,2, Daniel Riveline^1,2

¹Laboratory of Cell Physics, Institut de Science et d'Ingénierie Supramoléculaires (ISIS), CNRS and Université de Strasbourg, ²Development and Stem Cells Program, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS and Université de Strasbourg

We report a device and a new method to study cells and embryos. Single cells are precisely ordered in microcavity arrays. Their 3D confinement is a step towards 3D environments encountered in physiological conditions and allows organelle orientation. By controlling cell shape, this setup minimizes variability reported in standard assays.