Consejo Superior de Investigaciones Científicas (CSIC-UAM)

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity

Carlos G. Briz¹, Marta Navarrete², José A. Esteban², Marta Nieto¹

¹Department for Molecular and Cellular Biology, Centro Nacional de Biotecnología (CNB-CSIC), ²Molecular Neurobiology Department, Centro de Biología Molecular "Severo Ochoa", Consejo Superior de Investigaciones Científicas (CSIC-UAM)

This manuscript provides protocols that use in utero electroporation (IUE) to describe the structural connectivity of neurons at the single-cell level and the excitability of fluorescently labeled neurons. Histology is used to characterize dendritic and axonal projections. Whole-cell recording in acute slices is used to investigate excitability.