JoVE Logo

Sign In

National Ilan University

4 ARTICLES PUBLISHED IN JoVE

image

Environment

Evaluating the Effect of Environmental Chemicals on Honey Bee Development from the Individual to Colony Level
Chong-Yu Ko 1, Yue-Wen Chen 1, Yu-Shin Nai 1
1Department of Biotechnology and Animal Science, National Ilan University

Herein we present a method to feed pesticide contaminated food to both an individual honey bee and a beehive colony. The procedure evaluates the pesticide effect on individual honey bees by in vivo feeding of basic larval diet and also on the natural condition of beehive colony.

image

Genetics

Transient Expression of Foreign Genes in Insect Cells (sf9) for Protein Functional Assay
Ju-Chun Chang 1, Se Jin Lee 2, Jae Su Kim 2, Chung-Hsiung Wang 3, Yu-Shin Nai 1
1Department of Biotechnology and Animal Science, National Ilan University, 2Department of Agricultural Biology, College of Agriculture Life Sciences, Chonbuk National University, 3Department of Entomology, National Taiwan University

This protocol describes a heat shock-induced protein expression system (pDHsp/V5-His/sf9 cell system), which can be used for either expressing foreign proteins or evaluating the anti-apoptotic activity of potential foreign proteins and their truncated amino acids in insect cells.

image

Biochemistry

Extraction and Analysis of Taiwanese Green Propolis
Chun-Ting Chen 1, Yi-Hsuan Chien 1, Yu-Hsiang Yu 1, Yue-Wen Chen 1
1Department of Biotechnology and Animal Science, National Ilan University

We present a protocol for using ethanol as a solvent to extract and characterize Taiwanese green propolis that exhibits antibacterial activity.

image

Biology

Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests
Yao-Chia Liu 1, Nian-Tong Ni 1, Ju-Chun Chang 1, Yi-Hsuan Li 1, Mi Rong Lee 2, Jae Su Kim 2,3, Yu-Shin Nai 1
1Department of Entomology, National Chung Hsing University, 2Department of Agricultural biology, Jeonbuk National University, 3Department of Agricultural Convergence Technology, Jeonbuk National University

Here we present a protocol based on the mealworm (Tenebrio molitor)-bait system that was used for isolating and selecting entomopathogenic fungi (EPF) from soil samples. An effective conidia number (ECN) formula is used to select high stress tolerant EPF based on physiological characteristics for pest microbial control in the field.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved