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German Centre for Infection Research (DZIF)

5 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

An Experimental Model to Study Tuberculosis-Malaria Coinfection upon Natural Transmission of Mycobacterium tuberculosis and Plasmodium berghei
Ann-Kristin Mueller 1, Jochen Behrends 2, Jannike Blank 2, Ulrich E. Schaible 2, Bianca E. Schneider 2
1Department of Infectious Diseases, Parasitology Unit, University Hospital Heidelberg, 2Priority Area Infections, Research Center Borstel

Tuberculosis and malaria are two of the most prevalent infections in humans and major causes of morbidity and mortality in impoverished populations in the tropics. We established an experimental model system to study outcome of malaria-tuberculosis coinfection in mice after challenge with both pathogens via their natural route of infection.

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Medicine

In Vivo Tracking of Edema Development and Microvascular Pathology in a Model of Experimental Cerebral Malaria Using Magnetic Resonance Imaging
Angelika Hoffmann 1,2, Xavier Helluy 2,3, Manuel Fischer 2, Ann-Kristin Mueller 4,5, Sabine Heiland 2, Mirko Pham 1,6, Martin Bendszus 1, Johannes Pfeil 4,5,7
1Department of Neuroradiology, Heidelberg University Hospital, 2Division of Experimental Radiology, Department of Neuroradiology, Heidelberg University Hospital, 3NeuroImaging Centre Research, Department of Neuroscience, Ruhr-University Bochum, 4Centre for Infectious Diseases, Parasitology Unit, Heidelberg University Hospital, 5German Centre for Infection Research (DZIF), 6Department of Neuroradiology, University of Würzburg, 7Center for Childhood and Adolescent Medicine, General Pediatrics, Heidelberg University Hospital

We describe a mouse model of experimental cerebral malaria and show how inflammatory and microvascular pathology can be tracked in vivo using magnetic resonance imaging.

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Biology

Forced Salivation As a Method to Analyze Vector Competence of Mosquitoes
Anna Heitmann *1, Stephanie Jansen *1,2, Renke Lühken 1, Mayke Leggewie 1,2, Jonas Schmidt-Chanasit 1,2, Egbert Tannich 1,2
1Bernhard Nocht Institute for Tropical Medicine, 2German Centre for Infection Research (DZIF)

For efficient control of mosquito borne virus transmission, the knowledge of the vector potential of respective mosquitoes is of particular interest. We describe forced salivation as a method to analyze vector competence of Aedes albopictus and three different Culex taxa for the transmission of Zika virus.

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Bioengineering

Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry
Katarina Cirnski *1,2, Janetta Coetzee *1,2, Jennifer Herrmann 1,2, Rolf Müller 1,2
1Helmholtz Institute for Pharmaceutical Research Saarland, Department of Microbial Natural Products, Helmholtz Centre for Infection Research and Department of Pharmacy, Saarland University, 2Partner Site Hannover-Braunschweig, German Centre for Infection Research (DZIF)

The elucidation of the mode-of-action of a novel antibiotic is a challenging task in the drug discovery process. The goal of the method described here is the application of isothermal microcalorimetry using calScreener in antibacterial profiling to provide additional insight into drug-microbe interactions.

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Immunology and Infection

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test
Julia Häring *1, Tanja Michel *1, Matthias Becker 1, Daniel Junker 1, Tatia Tchitchagua 2, Olaf Leschnik 2, Berit Lange 3,4, Stefanie Castell 3,4, Gérard Krause 3,4, Monika Strengert 3, Alex Dulovic 1, Nicole Schneiderhan-Marra 1
1NMI Natural and Medical Sciences Institute at the University of Tübingen, 2Department of Neurology, Sächsisches Krankenhaus Rodewisch, 3Department of Epidemiology, Helmholtz Centre for Infection Research, 4German Centre for Infection Research (DZIF)

A three-channel dual-reporter fluorescence flow analysis system was used to develop a bead-based multiplex immunoassay that simultaneously evaluates serum samples for IgG and IgM elicited against multiple antigens of different Borrelia species that cause Lyme borreliosis in Europe and North America.

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