Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy ProjectFrancis R. G. Amrit 1, Arjumand Ghazi 1
1Department of Pediatrics, University of Pittsburgh School of Medicine, Children's Hospital of Pittsburgh
Galaxy and DAVID have emerged as popular tools that allow investigators without bioinformatics training to analyze and interpret RNA-Seq data. We describe a protocol for C. elegans researchers to perform RNA-Seq experiments, access and process the dataset using Galaxy and obtain meaningful biological information from the gene lists using DAVID.