JoVE Logo

Sign In

Vanderbilt University and Medical Center

4 ARTICLES PUBLISHED IN JoVE

image

Biology

Harvesting and Preparing Drosophila Embryos for Electrophysiological Recording and Other Procedures
David E. Featherstone 1, Kaiyun Chen 1, Kendal Broadie 2
1Department of Biological Sciences, University of Illinois, 2Department of Biological Sciences, Vanderbilt University

This technique exposes the Drosophila embryonic neuromusculature for immunohistochemistry or electrophysiological recording. It is useful for studying early events in neuromuscular development or performing electrophysiology in mutants that cannot hatch.

image

Biology

Electrophysiological Recording in the Drosophila Embryo
Kaiyun Chen 1, David E. Featherstone 1, Kendal Broadie 2
1Department of Biological Sciences, University of Illinois, 2Department of Biological Sciences, Vanderbilt University

Electrophysiological recordings from Drosophila embryos allow analyses of developing muscle and neuron electrical properties, as well as characterization of functional synaptogenesis at the glutamatergic neuromuscular junction and central cholinergic and GABAergic synapses.

image

JoVE Journal

FM Dye Cycling at the Synapse: Comparing High Potassium Depolarization, Electrical and Channelrhodopsin Stimulation
Danielle L. Kopke 1, Kendal Broadie 2
1Department of Biological Sciences, Vanderbilt University, 2Departments of Biological Sciences, Pharmacology, Cell and Developmental Biology, Kennedy Center for Research on Human Development, Vanderbilt University and Medical Center

Synaptic vesicle (SV) cycling is the core mechanism of intercellular communication at neuronal synapses. FM dye uptake and release are the primary means of quantitatively assaying SV endo- and exocytosis. Here, we compare all the stimulation methods to drive FM1-43 cycling at the Drosophila neuromuscular junction (NMJ) model synapse.

image

Neuroscience

Experience-Dependent Remodeling of Juvenile Brain Olfactory Sensory Neuron Synaptic Connectivity in an Early-Life Critical Period
Nichalas Nelson *1, Vanessa Miller *1, Nicholas Baumann 1, Kendal Broadie 1,2,3,4,5
1Department of Biological Sciences, Vanderbilt University and Medical Center, 2Department of Cell and Developmental Biology, Vanderbilt University and Medical Center, 3Department of Pharmacology, Vanderbilt University and Medical Center, 4Kennedy Center for Research on Human Development, Vanderbilt University and Medical Center, 5Vanderbilt Brain Institute, Vanderbilt University and Medical Center

We describe here methods for inducing and analyzing olfactory experience-dependent remodeling of antennal lobe synaptic glomeruli in the Drosophila juvenile brain.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved