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São Paulo State University (UNESP)

6 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci
Marlise I. Klein 1, Jin Xiao 1,2, Arne Heydorn 3, Hyun Koo 1,4
1Center for Oral Biology, University of Rochester Medical Center, 2State Key Laboratory of Oral Diseases, Sichuan University, 3Department of General Medicine, Glostrup Hospital, Glostrup, Denmark, 4Department of Microbiology and Immunology, University of Rochester Medical Center

Biofilms formed on tooth surfaces are highly complex and exposed to constant innate and exogenous environmental challenges, which modulate their architecture, physiology and transcriptome. We developed a toolbox to examine the composition, structural organization and gene expression of oral biofilms, which can be adapted to other areas of biofilm research.

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Developmental Biology

Derivation and Differentiation of Canine Ovarian Mesenchymal Stem Cells
Amanda B.T. Hill 1,2, Jonathan E.B.T. Hill 1, Fabiana F. Bressan 3, Maria Angélica Miglino 4, Joaquim M. Garcia 1
1The School of Agricultural and Veterinary Studies (FCAV), Jaboticabal, São Paulo State University (UNESP), 2CRRF, Université de Montréal, 3FZEA, University of São Paulo, 4FMVZ, University of São Paulo

Herein, we describe a method for the isolation, expansion, and differentiation of mesenchymal stem cells from canine ovarian tissue.

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Chemistry

Niobium Oxide Films Deposited by Reactive Sputtering: Effect of Oxygen Flow Rate
Silvia L. Fernandes 1, Lucas J. Affonço 2, Roberto A. R. Junior 2, José H. D. da Silva 2, Elson Longo 1, Carlos F. de O. Graeff 2
1Chemistry Department, Federal University of São Carlos (UFSCAR), 2Physics Department, School of Sciences, São Paulo State University (UNESP)

Here, we present a protocol for niobium oxide films deposition by reactive sputtering with different oxygen flow rates for use as an electron transport layer in perovskite solar cells.

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JoVE Core

Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries
Sabrina M. Ribeiro *1, Érick D. O. Fratucelli *1, Júlia M. Fernandes *2, Paula C. P. Bueno 2,3, Alberto José Cavalheiro 2, Marlise I. Klein 1
1Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (UNESP), 2Department of Organic Chemistry, Institute of Chemistry, São Paulo State University (UNESP), 3Faculty of Pharmaceutical Sciences of Ribeiraão Preto (FCFRP-USP), Department of Physics and Chemistry, University of São Paulo

Natural products represent promising starting points for the development of new drugs and therapeutic agents. However, due to the high chemical diversity, finding new therapeutic compounds from plants is a challenging and time-consuming task. We describe a simplified approach to identify antimicrobial and antibiofilm molecules from plant extracts and fractions.

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Immunology and Infection

Curcuminoid-Mediated Antimicrobial Photodynamic Therapy on a Murine Model of Oral Candidiasis
Ewerton Garcia de Oliveira Mima 1, Ana Cláudia Pavarina 1, Cláudia Carolina Jordão 1, Sâmmea Martins Vieira 2, Lívia Nordi Dovigo 2
1Department of Dental Materials and Prosthodontics, School of Dentistry, Araraquara, São Paulo State University (UNESP), 2Department of Social Dentistry, School of Dentistry, Araraquara, São Paulo State University (UNESP)

This protocol describes the application of antimicrobial Photodynamic Therapy (aPDT) in a murine model of oral candidiasis. aPDT was performed using a water-soluble mixture of curcuminoids and blue LED light.

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Biology

Microbiota of Attine Ants' Gardens: Visualizing a Microbial Landscape by Scanning Electron Microscopy
Mariana Oliveira Barcoto 1, Raphael Affonso Pereira Silva 1, Elliot Watanabe Kitajima 2, Andre Rodrigues 1
1Institute of Biosciences, Rio Claro, Department of General and Applied Biology, São Paulo State University (UNESP), 2Plant Pathology and Nematology Department, Escola Superior de Agricultura Luiz de Queiroz, University of São Paulo (USP)

We propose an optimized Scanning Electron Microscopy protocol for visualizing highly heterogeneous and delicate samples containing plant and fungal biomass, together with microbiota and biofilm. This protocol allows describing the spatial dimensions of the microbiota organization.

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