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Munich University of Applied Sciences

1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
Tanja D. Becke 1,2,3, Stefan Ness 2, Stefanie Sudhop 1,3, Hermann E. Gaub 3, Markus Hilleringmann 2, Arndt F. Schilling *4, Hauke Clausen-Schaumann *1,3
1Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, 2FG Protein Biochemistry & Cellular Microbiology, Munich University of Applied Sciences, 3Center for Nano Science, Ludwig-Maximilians-Universität München, 4Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie, University Medical Center Göttingen

This protocol describes the covalent immobilization of proteins with a heterobifunctional silane coupling agent to silicon-oxide surfaces designed for the atomic force microscopy based single molecule force spectroscopy which is exemplified by the interaction of RrgA (pilus-1 tip adhesin of S. pneumoniae) with fibronectin.

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