August 20th, 2018
•This protocol describes the covalent immobilization of proteins with a heterobifunctional silane coupling agent to silicon-oxide surfaces designed for the atomic force microscopy based single molecule force spectroscopy which is exemplified by the interaction of RrgA (pilus-1 tip adhesin of S. pneumoniae) with fibronectin.
Related Videos
Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support
An In Vitro Single-Molecule Imaging Assay for the Analysis of Cap-Dependent Translation Kinetics
Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved