Ultra-long Read Sequencing for Whole Genomic DNA AnalysisLiang Gong 1, Chee-Hong Wong 1, Jennifer Idol 1, Chew Yee Ngan 1, Chia-Lin Wei 1
1Genome Technologies, Jackson Laboratory for Genomic Medicine
Long-read sequences greatly facilitate the assembly of complex genomes and characterization of structural variation. We describe a method to generate ultra-long sequences by nanopore-based sequencing platforms. The approach adopts an optimized DNA extraction followed by modified library preparations to generate hundreds of kilobase reads with moderate coverage from human cells.
