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Ultra-long Read Sequencing for Whole Genomic DNA Analysis

DOI :

10.3791/58954-v

10:34 min

March 15th, 2019

March 15th, 2019

21,330 Views

1Genome Technologies, Jackson Laboratory for Genomic Medicine

Long-read sequences greatly facilitate the assembly of complex genomes and characterization of structural variation. We describe a method to generate ultra-long sequences by nanopore-based sequencing platforms. The approach adopts an optimized DNA extraction followed by modified library preparations to generate hundreds of kilobase reads with moderate coverage from human cells.

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Ultra long Read Sequencing

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