Generation, Amplification, and Titration of Recombinant Respiratory Syncytial VirusesCamille Bouillier 1, Vincent Rincheval 1, Delphine Sitterlin 1, Sabine Blouquit-Laye 1, Aurore Desquesnes 1, Jean-François Eléouët 2, Elyanne Gault 1,3, Marie-Anne Rameix-Welti 1,3
1UMR 1173 Institut national de la santé et de la recherche médicale (INSERM), Université de Versailles St. Quentin, 2UR892 Institut national de la recherche agronomique (INRA), Unité de virologie et immunologie moléculaires, 3Assistance Publique–Hôpitaux de Paris (AP-HP), Hôpital Ambroise Paré, Laboratoire de Microbiologie
We describe a method for generating and amplifying genetically modified respiratory syncytial viruses (RSVs) and an optimized plaque assay for RSVs. We illustrate this protocol by creating two recombinant viruses that respectively allow quantification of RSV replication and live analysis of RSV inclusion bodies and inclusion bodies-associated granules dynamics.
