Generation of 3-D Collagen-based Hydrogels to Analyze Axonal Growth and Behavior During Nervous System DevelopmentVanessa Gil 1,2,3,4, José Antonio Del Río 1,2,3,4
1Molecular and Cellular Neurobiotechnology, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Parc Científic de Barcelona, 2Department of Cell Biology, Physiology, and Immunology, Universitat de Barcelona, 3Center for Networked Biomedical Research on Neurodegenerative Diseases (CIBERNED), 4Institute of Neuroscience, University of Barcelona
Here, we provide a method for analyzing the behavior of growing axons in 3D matrices, mimicking their natural development.