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Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid

1 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone
Ginés Ávila-Pérez 1, Jun-Gyu Park 1, Aitor Nogales 1, Fernando Almazán 2, Luis Martínez-Sobrido 1
1Department of Microbiology and Immunology, University of Rochester Medical Center, 2Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid

The recent epidemic of Zika virus highlights the importance of establishing reverse genetic approaches to develop vaccines and/or therapeutic strategies. Here, we describe the protocol to rescue an infectious recombinant Zika virus from a full-length cDNA clone assembled in a bacterial artificial chromosome under the control of the human cytomegalovirus immediate-early promoter.

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