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Université libre de Bruxelles (ULB)

5 ARTICLES PUBLISHED IN JoVE

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Chemistry

[(DPEPhos)(bcp)Cu]PF6: A General and Broadly Applicable Copper-Based Photoredox Catalyst
Hajar Baguia 1, Christopher Deldaele 1, Bastien Michelet 1, Jérôme Beaudelot 1,2, Cédric Theunissen 1, Cécile Moucheron 2, Gwilherm Evano 1
1Laboratoire de Chimie Organique, Service de Chimie et PhysicoChimie Organiques, Université libre de Bruxelles (ULB), 2Laboratoire de Chimie Organique et Photochimie, Service de Chimie et PhysicoChimie Organiques, Université libre de Bruxelles (ULB)

Detailed and general protocols are presented for the synthesis of [(DPEPhos)(bcp)Cu]PF6, a general copper-based photoredox catalyst, and for its use in synthetic chemistry for the direct arylation of C-H bonds in (hetero)arenes and radical cyclization of organic halides.

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Biology

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method
Sema Elif Eski 1, Christine Dubois 2, Sumeet Pal Singh 1
1IRIBHM, Université Libre de Bruxelles (ULB), 2Medecine Faculty, Université Libre de Bruxelles (ULB)

Single nuclei isolation relies on dissociation and detergent-based permeabilization of the cell membrane, steps that need optimization and are prone to introducing technical artifacts. We demonstrate a detergent and enzyme-free protocol for rapid isolation of intact nuclei directly from whole tissue, yielding nuclei suitable for single-nucleus RNA-seq (snRNA-Seq) or ATAC-seq.

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Biology

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli
Thierry Oms *1, Tatjana Schlechtweg *1, Julien Cayron 1, Laurence Van Melderen 1
1Bacterial Genetics and Physiology, Département de Biologie Moléculaire, Faculté des Sciences, Université Libre de Bruxelles (ULB)

Antibiotic persistence describes the ability of small subpopulations within a sensitive isogenic population to transiently tolerate high doses of bactericidal antibiotics. The present protocol combines approaches to characterize the antibiotic persistence phenotype at the molecular and cellular levels after exposing Escherichia coli to lethal doses of ofloxacin.

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Developmental Biology

Gene Expression Analyses in Human Follicles
Melody Devos *1, Joana Dias Nunes *1, Isabelle Demeestere 1,2
1Research Laboratory on Human Reproduction, Université libre de Bruxelles (ULB), 2Fertility Clinic, Erasme Hospital, Université libre de Bruxelles (ULB)

Here, we describe a protocol outlining how to isolate human ovarian follicles from frozen-thawed cortical tissue to perform gene expression analyses.

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Immunology and Infection

Live Imaging and Characterization of Microglia Dynamics in the Zebrafish Embryo
Alice Montanari 1,2, Valérie Wittamer 1,2
1Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM), Université Libre de Bruxelles (ULB), 2ULB Institute of Neuroscience (UNI), Université Libre de Bruxelles (ULB)

We demonstrate a method that takes advantage of fast scanning confocal microscopy to perform live imaging of microglia cells in the developing zebrafish optic tectum, allowing for the analysis of the dynamics of these cells in vivo.

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