Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene TherapyVivek Bhaskaran 1, Pierpaolo Peruzzi 1
1Department of Neurosurgery, Brigham and Women's Hospital-Harvard Medical School
Described here is a protocol for characterizing modules of biologically synergistic miRNAs and their assembly into short transgenes, which allows simultaneous overexpression for gene therapy applications.