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Swiss Federal Institute of Technology Lausanne (EPFL)

2 ARTICLES PUBLISHED IN JoVE

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Biology

Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry
Mukul Girotra *1,2, Anne-Christine Thierry 3, Alexandre Harari 1,3, George Coukos 1, Olaia Naveiras 2,4, Nicola Vannini *1
1Department of Oncology UNIL CHUV, Ludwig Institute for Cancer Research Lausanne, University of Lausanne, 2Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology Lausanne (EPFL), 3Center of Experimental Therapeutics, Department of Oncology, Centre Hospitalier Universitaire Vaudois, 4Hematology Service, Department of Oncology, Centre Hospitalier Universitaire Vaudois (CHUV)

Here we describe a reliable method to measure mitochondrial mass and membrane potential in ex vivo cultured hematopoietic stem cells and T cells.

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Biology

Monitoring On-Target Signaling Responses in Larval Zebrafish - Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites
Kuan-Ting Huang 1, Phillippe Ly 1, Jesse R. Poganik 2, Saba Parvez 3, Marcus J. C. Long 4, Yimon Aye 1
1Swiss Federal Institute of Technology Lausanne (EPFL), 2Division of Genetics, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, 3Department of Pharmacology and Toxicology, College of Pharmacy, University of Utah, 4University of Lausanne (UNIL)

Zebrafish targeting reactive electrophiles and oxidants (Z-REX) is a chemical biology-based method for the investigation of reactive small-molecule signaling. This technique can be applied to live fish of different developmental stages. Here, we couple standard assays in zebrafish with Z-REX for signaling pathway analysis.

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