Monitoring Influenza Virus Survival Outside the Host Using Real-Time Cell AnalysisThomas Labadie 1, Quentin Grassin 2, Christophe Batéjat 2, Jean-Claude Manuguerra 2, India Leclercq 2,3
1Department of Infection Biology, London School of Hygiene and Tropical Medicine, 2Institut Pasteur, Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence (CIBU), 3Cellule Pasteur, Université de Paris
Reported here is a protocol for the quantification of infectious viral particles using real-time monitoring of electrical impedance of infected cells. A practical application of this method is presented by quantifying influenza A virus decay under different physicochemical parameters mimicking environmental conditions.