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Institut National de la Santé et de la Recherche Médicale (Inserm) UMR1065

3 ARTICLES PUBLISHED IN JoVE

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Biology

Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging
Jérôme Gilleron 1, Cindy Meziat 1, André Sulen 2, Stoyan Ivanov 3, Jennifer Jager 1, David Estève 4, Catherine Muller 4, Jean-Francois Tanti 1, Mireille Cormont 1
1Université Côte d'Azur, Inserm UMR1065, C3M, Team "Cellular and Molecular Pathophysiology of Obesity", Nice, France, 2Integrated Cardio Metabolic Center, Department of Medicine, Karolinska Institutet, Stockholm, Sweden, 3Université Côte d'Azur, Inserm UMR1065, C3M, Team "Haematometabolism in Diseases", Nice, France, 4Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS, Toulouse, France

Here, we describe a simple, inexpensive and fast clearing method to resolve the 3D structure of both mouse and human white adipose tissue using a combination of markers to visualize vasculature, nuclei, immune cells, neurons, and lipid-droplet coat proteins by fluorescent imaging.

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Medicine

Lymphatic and Blood Network Analysis During Obesity
Rafael S Czepielewski *1, Alexandre Gallerand *2,3, Jérôme Gilleron 2,4, Narges Khedher 2,3, Gwendalyn J. Randolph 1, Stoyan Ivanov 2,3
1Department of Pathology and Immunology, Washington University School of Medicine, 2Université Côte d'Azur, 3Mediterranean Center of Molecular Medicine C3M, Team 13, Institut National de la Santé et de la Recherche Médicale (Inserm) UMR1065, 4Mediterranean Center of Molecular Medicine C3M, Team 7, Institut National de la Santé et de la Recherche Médicale (Inserm) UMR1065

Obesity is a growing global public health issue. It has been previously associated with lymphatic dysfunction, suggesting a vital crosstalk between the adipose tissue and the lymphatic system. Here, we propose an accessible methodology allowing the distinct labeling of blood and lymphatic vasculatures within the subcutaneous adipose tissue.

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Biology

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
Jennifer Jager 1, Mélanie Gaudfrin 1, Jérôme Gilleron 1, Mireille Cormont 1, Jean-François Tanti 1
1Université Côte d’Azur, Inserm, C3M, Team Cellular and Molecular Pathophysiology of Obesity and Diabetes, Nice, France

Presented here is a protocol to deliver oligonucleotides such as small-interfering RNA (siRNA), micro-RNA mimics (miRs), or anti-micro-RNA (anti-miR) into mature adipocytes to modulate protein and micro-RNA expression.

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