Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging

7.4K Views

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10:10 min

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August 19th, 2020

DOI :

10.3791/61640-v

August 19th, 2020

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Jérôme Gilleron¹, Cindy Meziat¹, André Sulen², Stoyan Ivanov³, Jennifer Jager¹, David Estève⁴, Catherine Muller⁴, Jean-Francois Tanti¹, Mireille Cormont¹

¹Université Côte d'Azur, Inserm UMR1065, C3M, Team "Cellular and Molecular Pathophysiology of Obesity", Nice, France, ²Integrated Cardio Metabolic Center, Department of Medicine, Karolinska Institutet, Stockholm, Sweden, ³Université Côte d'Azur, Inserm UMR1065, C3M, Team "Haematometabolism in Diseases", Nice, France, ⁴Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS, Toulouse, France

Summary

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Pathological Dysfunction

Chapters in this video

0:04

Introduction

0:35

Mouse and Human White Adipose Tissue Fixation, Permeabilization, and Saturation

2:59

Mouse and Human White Adipose Tissue Staining

5:28

Mouse and Human White Adipose Tissue Clearing

6:17

3D Confocal Imaging

7:23

3D Adipose Tissue Image Quantitative Result Extraction

7:57

Results: Representative Adipose Tissue Labeling and Analysis

9:34

Conclusion

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