Sign In

Pflanzenwissenschaften (IBG-2), Forschungszentrum Jülich

2 ARTICLES PUBLISHED IN JoVE

image

Environment

Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
Megan Garvey 1, Johannes Klinger *1, Holger Klose *1, Rainer Fischer 2, Ulrich Commandeur 1
1Institute for Molecular Biotechnology, Bio7, RWTH Aachen University, 2Fraunhofer Institute for Molecular Biology and Applied Ecology

Tobacco plants were used to produce a fungal cellulase, TrCel5A, via a transient expression system. The expression could be monitored using a fluorescent fusion protein, and the protein activity was characterized post-expression.

image

Chemistry

Fractionation of Lignocellulosic Biomass using the OrganoCat Process
Leonie Schoofs 1, Dennis Weidener 1, Ulrich Schurr 1, Holger Klose 1, Philipp M. Grande 1
1Institut für Bio- und Geowissenschaften, Pflanzenwissenschaften (IBG-2), Forschungszentrum Jülich

OrganoCat is a method for the pretreatment and fractionation of lignocellulose under mild conditions into lignin, fermentable sugars, and cellulose pulp. In a biogenic, biphasic solvent system of water and 2-methyltetrahydrofuran with 2,5-furancarboxylic acid as catalyst, the OrganoCat products are separated in situ for straightforward product recovery.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved