Icahn School of Medicine at Mount Sinai, Icahn Institute of Data Science and Genomic Technology

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Single-Cell Characterization of Calcium Influx and HIV-1 Infection using a Multiparameter Optofluidic Platform

Tracey Freeman¹, Christina Andreou², Robert P. Sebra^3,4, Kristin G. Beaumont², Talia H. Swartz¹

¹Department of Medicine, Division of Infectious Diseases, Icahn School of Medicine at Mount Sinai, ²Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, Icahn Institute of Data Science and Genomic Technology, ³Black Family Stem Cell Institute, Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, Icahn Institute of Data Science and Genomic Technology, ⁴Sema4, a Mount Sinai venture

Here, we present a protocol in which single cells are monitored for acute events and productive HIV-1 infection on a nanofluidic device. Imaging data define virus-host receptor interactions and signaling pathway dynamics. This is the first method for nanofluidic high-throughput longitudinal single-cell culture and imaging to study signaling kinetics and molecular interactions.