University of Alabama at Birmingham (UAB)

1 ARTICLES PUBLISHED IN JoVE

Medicine

Culture and Imaging of Human Nasal Epithelial Organoids

Zhongyu Liu^1,2, Justin D. Anderson^1,2, Jennifer Natt^1,2, Jennifer S. Guimbellot^1,2

¹Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham (UAB), ²Department of Pediatrics, Division of Pulmonary and Sleep Medicine, UAB

A detailed protocol is presented here to describe an in vitro organoid model from human nasal epithelial cells. The protocol has options for measurements requiring standard laboratory equipment, with additional possibilities for specialized equipment and software.