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Culture and Imaging of Human Nasal Epithelial Organoids

3.6K Views

13:20 min

December 17th, 2021

DOI :

10.3791/63064-v

December 17th, 2021


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Human Nasal Epithelial Organoids

Chapters in this video

0:04

Introduction

1:40

Cell Culture and Expansion of Nasal Epithelial Cells

4:23

Growth and Differentiation of Organoids in Slides and Culture Inserts

6:06

Isolation and Fixation of Organoids

7:36

Preparation and Isolation of Organoids for Histological Sectioning

8:57

Baseline Lumen Measurements

9:56

Results: Live and Fixed Imaging of the Airway Epithelial Organoids Derived from Nasal Epithelial Cells and Analysis Methods

12:21

Conclusion

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