Culture and Imaging of Human Nasal Epithelial Organoids
3.6K Views
•
13:20 min
•
December 17th, 2021
DOI :
December 17th, 2021
•Chapters in this video
0:04
Introduction
1:40
Cell Culture and Expansion of Nasal Epithelial Cells
4:23
Growth and Differentiation of Organoids in Slides and Culture Inserts
6:06
Isolation and Fixation of Organoids
7:36
Preparation and Isolation of Organoids for Histological Sectioning
8:57
Baseline Lumen Measurements
9:56
Results: Live and Fixed Imaging of the Airway Epithelial Organoids Derived from Nasal Epithelial Cells and Analysis Methods
12:21
Conclusion
Related Videos
12:08
Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
11.1K Views
08:57
Generating and Imaging Mouse and Human Epithelial Organoids from Normal and Tumor Mammary Tissue Without Passaging
2.7K Views
08:00
Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study
10.6K Views
10:38
Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface
36.9K Views
09:02
Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions
1.3K Views
10:08
Establishing a Three-Dimensional Coculture Module of Epithelial Cells Using Nanofibrous Membranes
247 Views
11:13
Collection, Expansion, and Differentiation of Primary Human Nasal Epithelial Cell Models for Quantification of Cilia Beat Frequency
3.9K Views
07:44
Mucociliary Epithelial Organoids from Xenopus Embryonic Cells: Generation, Culture and High-Resolution Live Imaging
4.6K Views
Generation and Characterization of Murine Oral Mucosal Organoid Cultures
2.0K Views
10:12
Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids
8.0K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved