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Centro Nacional de Investigaciones Cardiovasculares (CNIC)

3 ARTICLES PUBLISHED IN JoVE

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Neuroscience

In vivo Positron Emission Tomography to Reveal Activity Patterns Induced by Deep Brain Stimulation in Rats
Marta Casquero-Veiga 1, Nicolás Lamanna-Rama 2,3, Diego Romero-Miguel 2,3, Manuel Desco 1,2,3,4, María Luisa Soto-Montenegro 2,4,5
1Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Laboratorio de Imagen Médica, Instituto de Investigación Sanitaria Gregorio Marañón, 3Departamento de Bioingeniería e Ingeniería Aeroespacial, Universidad Carlos III de Madrid, 4CIBER de Salud Mental (CIBERSAM), 5High Performance Research Group in Physiopathology and Pharmacology of the Digestive System (NeuGut), Universidad Rey Juan Carlos

We describe a preclinical experimental method to evaluate metabolic neuromodulation induced by acute deep brain stimulation with in vivo FDG-PET. This manuscript includes all experimental steps, from stereotaxic surgery to the application of the stimulation treatment and the acquisition, processing, and analysis of PET images.

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Developmental Biology

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos
Tamara Gonzalez-Costa 1,2, Jose Luis de la Pompa 1,2, Joaquim Grego-Bessa 1,2
1Intercellular Signalling in Cardiovascular Development and Disease Laboratory, Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Ciber de Enfermedades Cardiovasculares

Classically, the endocardium of the mouse embryonic valve primordium has been analyzed using transversal, coronal, or sagittal sections. Our novel approach for en face, two-dimensional imaging of the endocardium at valvulogenic regions allows planar polarity and cell rearrangement analysis of the endocardium during valve development.

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Developmental Biology

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo
Miquel Sendra 1, Jorge Mañes 1, Jorge N. Domínguez 2,3, Miguel Torres 1
1Cardiovascular Regeneration Program, Centro Nacional de Investigaciones Cardiovasculares (CNIC), 2Cardiovascular Development Group, Department of Experimental Biology, University of Jaén, Jaén, 3Fundación Medina, Granada

We present a detailed protocol for mouse embryo culture and imaging that enables 3D + time imaging of cardiac progenitor cells. This video-toolkit addresses the key skills required for successful live imaging otherwise hard to acquire from text-only publications.

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