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Sanjay Gandhi Postgraduate Institute of Medical Sciences

1 ARTICLES PUBLISHED IN JoVE

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Genetics

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants
Shalini Soni 1, Shubhra Agarwal 1, Rakesh Aggarwal 2, Naga Suresh Veerapu 1
1Virology Section, Department of Life Sciences, Shiv Nadar University, 2Gastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences

The protocol describes a method for introducing controllable genetic diversity in the hepatitis C virus genome by combining full-length mutant RNA synthesis using error-prone PCR and reverse genetics. The method provides a model for phenotype selection and can be used for 10 kb long positive-sense RNA virus genomes.

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