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Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna (IZSLER)

1 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Automated Analysis of Intracellular Phenotypes of Salmonella Using ImageJ
Melissa Berni 1, Stefano Pongolini 1, Martina Tambassi 1
1Risk Analysis and Genomic Epidemiology Unit, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia-Romagna (IZSLER)

Salmonella invades and replicates inside intestinal epithelial cells both in Salmonella-specific vacuoles and free in the cytosol (hyper-replication). A high-throughput fluorescence microscopy-based protocol is described here to quantify the intracellular phenotypes of Salmonella by two complementary image analyses through ImageJ, reaching single-cell resolution and scoring.

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