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5 ARTICLES PUBLISHED IN JoVE

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Biology

Endothelial Cell Tube Formation Assay for the In Vitro Study of Angiogenesis
Katie L. DeCicco-Skinner 1, Gervaise H. Henry 1, Christophe Cataisson 2, Tracy Tabib 1, J. Curtis Gwilliam 1, Nicholas J. Watson 1, Erica M. Bullwinkle 1, Lauren Falkenburg 1, Rebecca C. O'Neill 1, Adam Morin 1, Jonathan S. Wiest 2
1Department of Biology, American University, 2Laboratory of Cancer Biology and Genetics, National Cancer Institute, NIH

The tube formation assay is a fast, quantifiable method for measuring in vitro angiogenesis. Endothelial cells are combined with conditioned media and plated on basement membrane extract. Tube formation occurs within hours and newly formed tubules easily quantified.

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Genetics

Novel Sequence Discovery by Subtractive Genomics
Kathryn C. Asalone 1, Megan M. Nelson 1, John R. Bracht 1
1Biology Department, American University

The purpose of this protocol is to use a combination of computational and bench research to find novel sequences that cannot be easily separated from a co-purifying sequence, which may be only partially known.

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Chemistry

Gold Nanoparticle Modified Carbon Fiber Microelectrodes for Enhanced Neurochemical Detection
Sanuja Mohanaraj 1, Pauline Wonnenberg 1, Brianna Cohen 1, He Zhao 1, Matthew R. Hartings 1, Shouzhong Zou 1, Douglas M. Fox 1, Alexander G. Zestos 1,2
1Department of Chemistry, American University, 2Center for Behavioral Neuroscience, American University

In this study, we modify carbon-fiber microelectrodes with gold nanoparticles to enhance the sensitivity of neurotransmitter detection.

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Behavior

The Three-Chamber Choice Behavioral Task using Zebrafish as a Model System
Cassie J. Rowe 1,2, Mikayla Crowley-Perry 1,3, Elizabeth McCarthy 1, Terry L. Davidson 2,4, Victoria P. Connaughton 1,2
1Department of Biology, American University, 2Center for Behavioral Neuroscience, American University, 3Department of Chemistry, American University, 4Department of Neuroscience, American University

We present a behavioral chamber designed to assess cognitive performance. We provide data showing that once acquired, zebrafish remember the task 8 weeks later. We also show that hyperglycemic zebrafish have altered cognitive performance, indicating that this paradigm is applicable to studies assessing cognition and memory.

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Neuroscience

Alternate Immersion in Glucose to Produce Prolonged Hyperglycemia in Zebrafish
Elizabeth McCarthy 1, Cassie J. Rowe 1,2, Mikayla Crowley-Perry 1,3, Victoria P. Connaughton 1
1Department of Biology, American University, 2Center for Behavioral Neuroscience, American University, 3Department of Chemistry, American University

This protocol noninvasively induces hyperglycemia in zebrafish for up to 8 weeks. Using this protocol, an in-depth study of the adverse effects of hyperglycemia can be made.

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