University of Leipzig

5 ARTICLES PUBLISHED IN JoVE

JoVE Core

High-Sensitivity Nuclear Magnetic Resonance at Giga-Pascal Pressures: A New Tool for Probing Electronic and Chemical Properties of Condensed Matter under Extreme Conditions

Thomas Meier¹, Jürgen Haase¹

¹Faculty of Physics and Earth Sciences, University of Leipzig

Nuclear magnetic resonance is one of the most important spectroscopic tools. Here, the development of a new approach under high pressure, currently up to 10.1 GPa, is presented. This opens a new window into condensed matter physics and chemistry, where high-pressure research is of great importance.

Medicine

Non-fluoroscopic Catheter Tracking for Fluoroscopy Reduction in Interventional Electrophysiology

Philipp Sommer¹, Simon Kircher¹, Sascha Rolf¹, Sergio Richter¹, Micha Doering¹, Arash Arya¹, Andreas Bollmann¹, Gerhard Hindricks¹

¹Department of Electrophysiology, Heart Center, University of Leipzig

Radiation exposure is an underestimated risk in complex ablation procedures. Here, we describe a protocol to significantly decrease fluoroscopy time and dosage for both the patient and the lab staff by using a novel non-fluoroscopic catheter visualization system.

Immunology and Infection

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain

Claudia Pösel^*1, Karoline Möller^*1, Johannes Boltze^1,2, Daniel-Christoph Wagner¹, Gesa Weise^1,3

¹Ischemia Research Unit, Fraunhofer Institute for Cell Therapy and Immunology, ²Fraunhofer Research Institution for Marine Biotechnology, University of Lubeck, ³Department of Neurology, University of Leipzig

Inflammation plays a central role in the pathogenesis of ischemic stroke. Increasing evidence suggests that it acts as a double-edged sword which exacerbates early brain injury, but also contributes to later repair. This protocol describes the isolation of immune cells from the ischemic brain and their subsequent flow cytometric phenotyping.

Immunology and Infection

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

Jörg Flemmig¹, Pauline Schwarz¹, Ingo Bäcker², Anna Leichsenring², Franziska Lange², Jürgen Arnhold¹

¹Institute for Medical Physics and Biophysics, Medical Faculty, University of Leipzig, ²Fraunhofer Institute for Cell Therapy and Immunology (IZI) Leipzig

This protocol describes the quick enrichment of leukocytes from small blood samples for a subsequent specific determination of the halogenating peroxidase activity within the cells. The method can be applied to human and non-human material and may contribute to the evaluation of new inflammatory markers.

Chemistry

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Robert Serfling^*1, Lisa Seidel^*1, Thore Böttke^*1, Irene Coin¹

¹Institute of Biochemistry, Faculty of Life Sciences, University of Leipzig

A facile fluorescence assay is presented to evaluate the efficiency of amino-acyl-tRNA-synthetase/tRNA pairs incorporating non-canonical amino-acids (ncAAs) into proteins expressed in mammalian cells. The application of ncAAs to study G-protein coupled receptors (GPCRs) is described, including photo-crosslinking mapping of binding sites and bioorthogonal GPCR labeling on live cells.