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Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

8.3K Views

14:02 min

April 9th, 2018

DOI :

10.3791/57069-v

April 9th, 2018


Transcript

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GPCR

Chapters in this video

0:04

Title

1:11

Fluorescence-based Screening of Incorporation Efficiencies

4:22

Genetic Incorporation of ncAAs into GPCRs

8:21

Ultrafast Bioorthogonal Labeling of GPCRs on Live Mammalian Cells

11:11

Results: Incorporation of Chemical Probes into GPCRs in Mammalian Cells for Cross-linking and Imaging Studies

12:52

Conclusion

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