Establishment of Cancer Stem Cell Cultures from Human Conventional OsteosarcomaGaia Palmini 1, Roberto Zonefrati 1, Carmelo Mavilia 1, Alessandra Aldinucci 2, Ettore Luzi 1, Francesca Marini 1, Alessandro Franchi 1, Rodolfo Capanna 3, Annalisa Tanini 1, Maria Luisa Brandi 1
1Department of Surgery and Translational Medicine (DCMT), University of Florence, 2Neurofarba Department, University of Florence, 3Department of Traumatology and General Orthopedics, Azienda Ospedaliera Universitaria Careggi
The presence of cancer stem cells (CSCs) in bone sarcomas has recently been linked to their pathogenesis. In this article, we present the isolation of CSCs from primary cell cultures obtained from human biopsies of conventional osteosarcoma (OS) using the ability of CSCs to grow under nonadherent conditions.
