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Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma

DOI :

10.3791/53884-v

9:25 min

October 14th, 2016

October 14th, 2016

18,283 Views

1Department of Surgery and Translational Medicine (DCMT), University of Florence, 2Neurofarba Department, University of Florence, 3Department of Traumatology and General Orthopedics, Azienda Ospedaliera Universitaria Careggi

The presence of cancer stem cells (CSCs) in bone sarcomas has recently been linked to their pathogenesis. In this article, we present the isolation of CSCs from primary cell cultures obtained from human biopsies of conventional osteosarcoma (OS) using the ability of CSCs to grow under nonadherent conditions.

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Cancer Stem Cells

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