JoVE Logo
Faculty Resource Center

Sign In

Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma

DOI :

10.3791/53884-v

9:25 min

October 14th, 2016

October 14th, 2016

18,268 Views

1Department of Surgery and Translational Medicine (DCMT), University of Florence, 2Neurofarba Department, University of Florence, 3Department of Traumatology and General Orthopedics, Azienda Ospedaliera Universitaria Careggi

The presence of cancer stem cells (CSCs) in bone sarcomas has recently been linked to their pathogenesis. In this article, we present the isolation of CSCs from primary cell cultures obtained from human biopsies of conventional osteosarcoma (OS) using the ability of CSCs to grow under nonadherent conditions.

Tags

Cancer Stem Cells

-- Views

Related Videos

article

Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells

article

Real-Time Monitoring of Human Glioma Cell Migration on Dorsal Root Ganglion Axon-Oligodendrocyte Co-Cultures

article

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines

article

Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells

article

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

article

Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures

article

In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System

article

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells

article

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples

article

Expansion and Enrichment of Gamma-Delta (γδ) T Cells from Apheresed Human Product

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved