Babraham Research Campus

2 ARTICLES PUBLISHED IN JoVE

Genetics

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Stefan Schoenfelder^*1, Biola-Maria Javierre^*1,2, Mayra Furlan-Magaril^1,3, Steven W. Wingett^1,4, Peter Fraser^1,5

¹Nuclear Dynamics Programme, The Babraham Institute, Babraham Research Campus, ²IJC Building, Campus ICO-Germans Trias i Pujol, Josep Carreras Leukemia Research Institute, ³Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, ⁴Bioinformatics Group, The Babraham Institute, Babraham Research Campus, ⁵Department of Biological Science, Florida State University

DNA regulatory elements, such as enhancers, control gene expression by physically contacting target gene promoters, often through long-range chromosomal interactions spanning large genomic distances. Promoter Capture Hi-C (PCHi-C) identifies significant interactions between promoters and distal regions, enabling the assignment of potential regulatory sequences to their target genes.

Genetics

In-Nucleus Hi-C in Drosophila Cells

Ayerim Esquivel-López¹, Rodrigo Arzate-Mejía¹, Rosario Pérez-Molina¹, Mayra Furlan-Magaril¹

¹Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México

The genome is organized in the nuclear space into different structures that can be revealed through chromosome conformation capture technologies. The in-nucleus Hi-C method provides a genome-wide collection of chromatin interactions in Drosophila cell lines, which generates contact maps that can be explored at megabase resolution at restriction fragment level.