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Center for Animal Health Research, INIA-CISA

3 ARTICLES PUBLISHED IN JoVE

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JoVE Journal

Influenza A Virus Studies in a Mouse Model of Infection
Laura Rodriguez 1, Aitor Nogales 1, Luis Martínez-Sobrido 1
1Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry

Influenza A viruses (IAVs) are important human respiratory pathogens. To understand the pathogenicity of IAVs and to perform preclinical testing of novel vaccine approaches, animal models mimicking human physiology are required. Here, we describe techniques to evaluate IAV pathogenesis, humoral responses and vaccine efficacy using a mouse model of infection.

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Immunology and Infection

Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone
Ginés Ávila-Pérez 1, Jun-Gyu Park 1, Aitor Nogales 1, Fernando Almazán 2, Luis Martínez-Sobrido 1
1Department of Microbiology and Immunology, University of Rochester Medical Center, 2Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid

The recent epidemic of Zika virus highlights the importance of establishing reverse genetic approaches to develop vaccines and/or therapeutic strategies. Here, we describe the protocol to rescue an infectious recombinant Zika virus from a full-length cDNA clone assembled in a bacterial artificial chromosome under the control of the human cytomegalovirus immediate-early promoter.

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Immunology and Infection

A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection
Kevin Chiem 1, Javier Rangel-Moreno 2, Aitor Nogales 1,3, Luis Martinez-Sobrido 1
1Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 2Division of Allergy/Immunology and Rheumatology, Department of Medicine, University of Rochester, 3Center for Animal Health Research, INIA-CISA

Influenza A viruses (IAVs) are contagious respiratory pathogens that cause annual epidemics and occasional pandemics. Here, we describe a protocol to track viral infections in vivo using a novel recombinant luciferase and fluorescence-expressing bi-reporter IAV (BIRFLU). This approach provides researchers with an excellent tool to study IAV in vivo.

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