Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and BundlesHugo Wioland 1, Foad Ghasemi 1, Jahnavi Chikireddy 1, Guillaume Romet-Lemonne 1, Antoine Jégou 1
1Université Paris Cité, CNRS, Institut Jacques Monod
We present protocols for simple actin filament microfluidic assays, in combination with fluorescence microscopy, that allow one to accurately monitor individual actin filaments in real-time while sequentially exposing them to different protein solutions.
