Configuring the Setup with Standard Flow Rates and Changing Solution
4:21
Basic Single Filament Experiment: Adenosine Diphosphate (ADP)-Actin Barbed End Depolymerization
6:45
Results: Polymerization of Actin Filaments from Spectrin-Actin Seeds when Exposed to Different Actin Binding Proteins
7:24
Conclusion
Transcript
We combine fluorescence microscopy with microfluidics to study how actin-binding proteins regulate the assembly, and the disassembly, of actin filaments. With microfluidics, we are able to quantify reactions happening on tens of individual actin f
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We present protocols for simple actin filament microfluidic assays, in combination with fluorescence microscopy, that allow one to accurately monitor individual actin filaments in real-time while sequentially exposing them to different protein solutions.