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Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles

DOI :

10.3791/63891-v

May 5th, 2022

May 5th, 2022

2,230 Views

1Université Paris Cité, CNRS, Institut Jacques Monod

We present protocols for simple actin filament microfluidic assays, in combination with fluorescence microscopy, that allow one to accurately monitor individual actin filaments in real-time while sequentially exposing them to different protein solutions.

Tags

Microfluidics

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