New York University Grossman School of Medicine

3 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Is My Mouse Pregnant? High-Frequency Ultrasound Assessment

¹Department of Pediatrics, New York University Grossman School of Medicine, ²Department of Anesthesiology, New York University Grossman School of Medicine, ³Department of Cell Biology, New York University Grossman School of Medicine

High-resolution ultrasound can help streamline experiments requiring timed-pregnant mice by determining the state of pregnancy, gestational age, and pregnancy losses. Presented here is a protocol to illustrate methods to assess mouse pregnancies as well as potential pitfalls (image artifacts) that may mimic pregnancy.

Neuroscience

Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration

Jeffrey D. Messinger¹, Max Brinkmann², James A. Kimble¹, Andreas Berlin¹, K. Bailey Freund^3,4, Gregory H. Grossman⁵, Thomas Ach^*6, Christine A. Curcio^*1

¹Department of Ophthalmology and Visual Sciences, University of Alabama at Birmingham Heersink School of Medicine, ²Department of Ophthalmology, University Hospital of Zurich, ³Vitreous Retina Macula Consultants of New York, ⁴Department of Ophthalmology, New York University Grossman School of Medicine, ⁵Advancing Sight Network, ⁶Department of Ophthalmology, University Hospital Bonn

Laboratory assays can leverage prognostic value from the longitudinal optical coherence tomography (OCT)-based multimodal imaging of age-related macular degeneration (AMD). Human donor eyes with and without AMD are imaged using OCT, color, near-infrared reflectance scanning laser ophthalmoscopy, and autofluorescence at two excitation wavelengths prior to tissue sectioning.

Chemistry

Enhancing Efficiency and Radiolabeling Yields of Carbon-11 Radioligands for Clinical Research Using the Loop Method

Jairo Baquero¹, Simon K. Joseph¹, Jakub Mroz¹, Camry V. A'Keen¹, Melchor V. Cantorias¹, Briana M. Roman¹, Patrick Carberry¹

¹Department of Radiology, New York University Grossman School of Medicine

This protocol presents a detailed procedure to prepare four carbon-11 radiotracers by way of the “loop method” [¹¹C]methylation. We describe the procedure to synthesize [¹¹C]ER-176, with data for three additional radiotracers: [¹¹C]MRB, [¹¹C]mHED, and [¹¹C]PiB. The loop method provides an efficient synthesis with increased radiochemical yields against traditional reaction vessel [¹¹C]methylation.