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Northwestern University, Feinberg School of Medicine

3 ARTICLES PUBLISHED IN JoVE

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Bioengineering

A Method for Ovarian Follicle Encapsulation and Culture in a Proteolytically Degradable 3 Dimensional System
Ariella Shikanov 1, Min Xu 2, Teresa K. Woodruff 2,3,4, Lonnie D. Shea 1,4,5
1Institute for BioNanotechnology in Advanced Medicine, Northwestern University, 2Department of Obstetrics and Gynecology, Northwestern University, Feinberg School of Medicine, 3Center for Reproductive Research, Northwestern University, 4The Robert H. Lurie Comprehensive Cancer Center, Northwestern University, 5Department of Chemical and Biological Engineering, Northwestern University

A new method for ovarian follicle encapsulation in a 3D fibrin-alginate interpenetrating network is described. This system combines structural support with proteolytic degradation to support the development of immature follicles to produce mature oocytes. This method may be applied to culture cell aggregates to maintain cell-cell contacts without limiting expansion.

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Neuroscience

Purkinje Cell Survival in Organotypic Cerebellar Slice Cultures
Jennifer Rakotomamonjy 1, Alicia Guemez-Gamboa 1
1Department of Physiology, Northwestern University, Feinberg School of Medicine

Organotypic slice cultures are a powerful tool to study neurodevelopmental or degenerative/regenerative processes. Here, we describe a protocol that models the neurodevelopmental death of Purkinje cells in mouse cerebellar slice cultures. This method may benefit research in neuroprotective drug discovery.

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Cancer Research

Automated Dissection Protocol for Tumor Enrichment in Low Tumor Content Tissues
Charles A. Havnar 1, Oliver Zill 2, Jeff Eastham 1, Jeffrey Hung 1, Manana Javey 3, Emmanuel Naouri 3, Jennifer Giltnane 1, Justin M. Balko 4, Andrew Wallace 2, Nicolas Lounsbury 2, Daniel Oreper 2, Sarajane Saturnio 1, G-Y Yang 5, Amy A. Lo 1
1Departments of Research Pathology, Genentech, 2Bioinformatics & Computational Biology, Genentech, 3Roche Sequencing Solutions, Hacienda Drive, 4Department of Medicine, Vanderbilt University Medical Center, Medical Center Drive, 5Department of Pathology, Northwestern University, Feinberg School of Medicine

Digital annotation with automated tissue dissection provides an innovative approach to enriching tumor in low tumor content cases and is adaptable to both paraffin and frozen tissue types. The described workflow improves accuracy, reproducibility and throughput and could be applied to both research and clinical settings.

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