A Method for Ovarian Follicle Encapsulation and Culture in a Proteolytically Degradable 3 Dimensional System

24.3K Views

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12:37 min

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March 15th, 2011

DOI :

10.3791/2695-v

March 15th, 2011

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Ariella Shikanov¹, Min Xu², Teresa K. Woodruff²^,³^,⁴, Lonnie D. Shea¹^,⁴^,⁵

¹Institute for BioNanotechnology in Advanced Medicine, Northwestern University, ²Department of Obstetrics and Gynecology, Northwestern University, Feinberg School of Medicine, ³Center for Reproductive Research, Northwestern University, ⁴The Robert H. Lurie Comprehensive Cancer Center, Northwestern University, ⁵Department of Chemical and Biological Engineering, Northwestern University

Summary

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Ovarian Follicle

Encapsulation

Culture

Proteolytically Degradable

3 Dimensional System

In Vitro Follicle Techniques

Hydrogels

Native Ovarian Environment

3D Follicular Architecture

Cell cell Interactions

Paracrine Signaling

Alginate

Inert Algae derived Polysaccharide

Gelation

Calcium Ions

Developmental Competence

Degradable

Fibrin alginate Interpenetrating Network FA IPN

Dynamic Mechanical Environment

Chapters in this video

0:05

Title

3:02

Follicle Isolation

5:11

The Drop Method

7:16

The Parafilm Method

8:31

Follicle Recovery from the 3D Matrix

10:00

Alginate and Fibrin-alginate for 3D Follicle Culture in Vitro

11:48

Conclusion

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